[HTML][HTML] Lyse-Reseal Erythrocytes for Transfection of Plasmodium falciparum

G Govindarajalu, Z Rizvi, D Kumar, PS Sijwali - Scientific reports, 2019 - nature.com
Simple and efficient transfection methods for genetic manipulation of Plasmodium
falciparum are desirable to identify, characterize and validate the genes with therapeutic
potential and better understand parasite biology. Among the available transfection
techniques for P. falciparum, electroporation-based methods, particularly electroporation of
ring-infected RBCs is routinely used. Nonetheless, transfection of P. falciparum remains a
resource-intensive procedure. Here, we report a simple and economic transfection method …

Lyse-reseal erythrocytes for transfection of Plasmodium falciparum.

GG Gokulapriya Govindarajalu, ZR Zeba Rizvi… - 2019 - cabidigitallibrary.org
Simple and efficient transfection methods for genetic manipulation of Plasmodium
falciparum are desirable to identify, characterize and validate the genes with therapeutic
potential and better understand parasite biology. Among the available transfection
techniques for P. falciparum, electroporation-based methods, particularly electroporation of
ring-infected RBCs is routinely used. Nonetheless, transfection of P. falciparum remains a
resource-intensive procedure. Here, we report a simple and economic transfection method …
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